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Molecular Dynamics Inc phosphorimager analysis
Phosphorimager Analysis, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphorimager+analysis/phosphorimager+%C5%BE%C3%B8/pm15740086__ja045364wsi20050112_095109-7-8-12
Average 86 stars, based on 1 article reviews
phosphorimager analysis - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Extraction:

Article Title: Transcriptional and translational S-box riboswitches differ in ligand-binding properties
Article Snippet: An antisense oligonucleotide (TCCCCCT) complementary to the SD region (nts 180-186) was added to SAM-bound and SAM-free RNA (40 nM) at 100-fold molar excess and the reactions were incubated at 37°C for 5 min. RNase H (10 U/μL; Ambion) was added and the reactions were incubated at 37°C for 10 min. .. The reactions were terminated by phenolchloroform extraction, and products were resolved by denaturing 6% (wt/vol) PAGE and visualized by PhosphorImager analysis (Molecular Dynamics). .. A DNA primer complementary to the positions 277-302 of the 3’ end of the D. indicum metI transcript was 5′ end-labeled (54) with [γ-32P]-ATP (6000 Ci/mmol, Perkin Elmer).

Article Title: Transcriptional and translational S-box riboswitches differ in ligand-binding properties
Article Snippet: An antisense oligonucleotide (5′-TCCCCCT-3′) complementary to the SD region (nucleotides 180–186) was added to SAM-bound and SAM-free RNA (40 n m ) at 100-fold molar excess, and the reactions were incubated at 37 °C for 5 min. RNase H (10 units/μl, Ambion) was added, and the reactions were incubated at 37 °C for 10 min. .. The reactions were terminated by phenol–chloroform extraction, and products were resolved by denaturing 6% (w/v) PAGE and visualized by PhosphorImager analysis (Molecular Dynamics). .. A DNA primer complementary to the positions 277–302 of the 3′ end of the D. indicum metI transcript was 5′ end–labeled ( 54 ) with [γ- 32 P]ATP (6000 Ci/mmol, PerkinElmer Life Sciences).

Polyacrylamide Gel Electrophoresis:

Article Title: Transcriptional and translational S-box riboswitches differ in ligand-binding properties
Article Snippet: An antisense oligonucleotide (TCCCCCT) complementary to the SD region (nts 180-186) was added to SAM-bound and SAM-free RNA (40 nM) at 100-fold molar excess and the reactions were incubated at 37°C for 5 min. RNase H (10 U/μL; Ambion) was added and the reactions were incubated at 37°C for 10 min. .. The reactions were terminated by phenolchloroform extraction, and products were resolved by denaturing 6% (wt/vol) PAGE and visualized by PhosphorImager analysis (Molecular Dynamics). .. A DNA primer complementary to the positions 277-302 of the 3’ end of the D. indicum metI transcript was 5′ end-labeled (54) with [γ-32P]-ATP (6000 Ci/mmol, Perkin Elmer).

Article Title: Transcriptional and translational S-box riboswitches differ in ligand-binding properties
Article Snippet: An antisense oligonucleotide (5′-TCCCCCT-3′) complementary to the SD region (nucleotides 180–186) was added to SAM-bound and SAM-free RNA (40 n m ) at 100-fold molar excess, and the reactions were incubated at 37 °C for 5 min. RNase H (10 units/μl, Ambion) was added, and the reactions were incubated at 37 °C for 10 min. .. The reactions were terminated by phenol–chloroform extraction, and products were resolved by denaturing 6% (w/v) PAGE and visualized by PhosphorImager analysis (Molecular Dynamics). .. A DNA primer complementary to the positions 277–302 of the 3′ end of the D. indicum metI transcript was 5′ end–labeled ( 54 ) with [γ- 32 P]ATP (6000 Ci/mmol, PerkinElmer Life Sciences).

Radioactivity:

Article Title: Human Apurinic/Apyrimidinic Endonuclease (APE1) Is Acetylated at DNA Damage Sites in Chromatin, and Acetylation Modulates Its DNA Repair Activity
Article Snippet: .. The gels were dried, and the radioactivity was quantitated by PhosphorImager analysis in a Storm system (Molecular Dynamics). ..

Software:

Article Title: Transcriptional and translational S-box riboswitches differ in ligand-binding properties
Article Snippet: .. Products were visualized using PhosphorImager analysis (Molecular Dynamics) and quantified using ImageQuant 5.2 software. ..

Article Title: Transcriptional and translational S-box riboswitches differ in ligand-binding properties
Article Snippet: .. Products were visualized by using PhosphorImager analysis (Molecular Dynamics) and quantified by using ImageQuant 5.2 software. ..

Autoradiography:

Article Title: The Mimivirus L375 Nudix enzyme hydrolyzes the 5' mRNA cap.
Article Snippet: 2 μl aliquots of each reaction were resolved on a polyethyleneimine-cellulose thin layer chromatography plate (Sigma-Aldrich) developed in 0.75 M LiCl. .. UV shadowing was used to detect unlabeled nucleotide standards while autoradiography and PhosphorImager analysis (Molecular Dynamics) were used to visualize radioactive signals. ..

other:

Article Title: An In Vitro Selection System for TNA
Article Snippet: Gels from Supporting figure 1 were quantified by phosphorimager analysis (ImageQuant v1.2, Molecular Dynamics).



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